The biological buffering agent DIPSO, also known as 3- [N, N-di (hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, has a CAS number of 68399-80-4. It appears as a white crystalline powder and is a zwitterionic buffering agent. At room temperature, the pH value of 0.1M DIPSO powder in water is between 4.0-5.5, indicating an acidic liquid; The acidity coefficient pKa has a value of 7.6, and the pH value range of the acid-base indicator for color change is between 7.0-8.2. It is widely used in scientific research and chemical industry. 1. The advantages of DIPSO As a member of the zwitterionic buffer, DIPSO has certain improvements in water solubility, chemical stability, and compatibility with other biological agents compared to traditional phosphate buffering agents. In addition, DIPSO has resistance to enzymatic and non enzymatic degradation, and is less affected by medium concentration, temperature, and ion composition. Not only is it less toxic to cells, but the pKa value is similar to the pH value of organisms, making it highly compatible with biological systems. 2. Application of DIPSO DIPSO has a wide range of uses and is a commonly used buffer in biochemical separation and purification analysis, used to stabilize the pH value of the required liquid in experiments; It can also be used in electrophoresis experiments to provide the required ions and ensure the stability and repeatability of the migration process; It can also be combined with other buffering agents such as HEPES, MOPS, etc. to form new buffering agents, expand the pH high activity range of the buffer, and thereby reduce the required buffering agent concentration. 3. Precautions for use The original shape of DIPSO is white crystalline powder, which needs to be prepared into a solution in a certain proportion during use. Protective tools should be worn during operation to avoid direct contact with human skin, eyes, ears, and nose; The aqueous solution of DIPSO is transparent and does not differ significantly from water to the naked eye. Therefore, the prepared DIPSO solution should be promptly labeled to distinguish it. In addition, when storing DIPSO, it is advisable to seal it in a dry, ventilated, and light proof environment. DIPSO buffer is not commonly available on the market at present. If you want to purchase it, please contact Hubei Xindesheng Material Technology Co., Ltd. The DIPSO produced by Desheng has high purity and good water solubility; Stable production process, small inter batch differences, professional R&D team, and guaranteed product quality!