1、 Chemical properties The chromogenic substrate DA67, CAS number 115871-18-6, with a molecular weight of 408.45, appears as a white crystalline powder that is soluble in water. Its chromogenic reaction is usually produced by reacting with peroxidase. DA67 is compatible with water solubility and high sensitivity for the first time. Previously, it had to be achieved through the use of luminescent or fluorescent reagents, but now it can be achieved using DA67 without the need for other luminescent agents. 2、 Application field Due to its ability to obtain results directly using a colorimeter, it is suitable for detecting samples of biological components, such as protein electrophoresis, nucleic acid electrophoresis, and other experiments as a substrate. By measuring the time consumed by substrates and changes in absorbance, information such as enzyme activity and substrate concentration can be reflected. 3、 Performance characteristics 1. High sensitivity: DA67 has high sensitivity and can detect small changes in substrate consumption, which makes it widely used in biochemical experiments and molecular biology. 2. Good repeatability: The color reaction of DA67 has good repeatability and can be quantitatively analyzed through standardized methods, which makes the experimental results more accurate and reliable. 3. High water solubility: Traditional water-soluble color reagents, due to the low molecular absorption coefficient of color pigments, are prone to the phenomenon of being slightly soluble and difficult to handle. However, DA67 has high water solubility and can be directly soluble in water at concentrations above 1mmol/L. 4、 Precautions 1. DA67 is easily affected by light and heat, so it is necessary to avoid prolonged exposure to air or high temperature environments when storing and using it. It is recommended to store it in a cool and dry place and use it as soon as possible. 2. The color reaction of DA67 is influenced by various factors, including substrate concentration, enzyme activity, reaction temperature, etc. Therefore, reasonable adjustments and controls need to be made according to experimental requirements during use. 3. When measuring the absorbance of DA67, it is necessary to calculate and select the appropriate detection wavelength to ensure the accuracy and reliability of the results, while also paying attention to calibrating the instrument and eliminating external interference.