Bicine Na is a biological buffer, also known as N, N-dihydroxyethylglycine sodium. It is one of the commonly used buffering agents in biological experiments, which has the function of adjusting the pH value of the solution. Bicine Na has a chemical structure similar to glycine, but the hydrogen on its amino nitrogen atom is replaced by two hydroxyethyl groups, which gives it good water solubility and stability. In biological experiments, Bicine Na can provide a stable pH environment to help maintain the acid-base balance of the reaction system, which is crucial for the smooth progress of many biochemical reactions. In addition, Bicine Na also has some other advantages. For example, it does not react with calcium and magnesium ions in biological systems, so it does not interfere with experimental results. Meanwhile, Bicine Na can chelate heavy metal ions such as copper, cadmium, and lead, thus having certain applications in the remediation of soil contaminated with heavy metals. When using Bicine Na, it is usually necessary to prepare it into a solution of a certain concentration. To ensure the accuracy of the experimental results, it is necessary to ensure that the instruments used are completely clean and avoid doping with other impurities. At the same time, it is necessary to strictly follow the proportion during preparation to ensure the accurate concentration and pH value of the solution.