CHES-Na is a commonly used biological buffer with the chemical formula CHES-Na. It is commonly used to regulate the pH value of biological reaction systems to maintain the activity of biological molecules. CHES-Na is a white crystalline powder that is easily soluble in water but insoluble in organic solvents. Its aqueous solution has high buffering capacity and can maintain the stability of the pH value of the biological reaction system. In biological experiments, CHES-Na is commonly used in experiments such as cell culture and protein crystallization to regulate the pH value of the experimental system and protect the activity of biological molecules. In addition, CHES-Na can also be used in electrophoresis experiments as a supporting electrolyte to maintain the stability of the pH value of the electrophoresis system. It should be noted that CHES-Na has a certain degree of irritation, and direct contact with skin and eyes should be avoided during use. At the same time, sufficient dissolution and dilution should be carried out before use to avoid affecting the experimental results.