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China A detailed introduction to mopso buffer - China Supplier
China A detailed introduction to mopso buffer - China Supplier

A detailed introduction to mopso buffer

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  • Add:Guanggu United Science and Technology City C8, Ezhou City, Hubei Province, Zip: 43603
  • Contact: vickyzhao
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  • Email:vickyzhao@whdschem.com

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Good's zwitterionic buffer is a buffer system developed by Good and his team in 1960. This series of buffers is a specific buffer system specifically developed for life science research, and MOPSO buffer is one of the Good's buffer series. The Chinese name of MOPSO is 3-morpholin-2-hydroxypropylsulfonic acid. Like other Good's buffers, it does not participate in or interfere with biochemical reaction processes and has no inhibitory effect on enzymatic reactions. Therefore, it is specifically used for the research of organelles and highly variable, pH sensitive proteins and enzymes. MOPSO has the following characteristics: 1. High solubility in water; 2. Difficult to penetrate biofilm; 3. Small salt effect; 4. The influence of ion concentration, solution composition, temperature, etc. on dissociation is small; 5. Not forming complexes or precipitates with metal ions; 6. Low absorption of light within the wavelength range of ultraviolet and visible light. Configuration method for MOPSO: 1. Prepare 800 mL of distilled water (dH2O) in a beaker; 2. 112.63 grams of MOPSO powder are put into a beaker containing distilled water (dH2O); 3. Adjust the solution in the beaker to the desired pH value using a 10% sodium hydroxide solution (10N NaOH); 4. Add distilled water (dH2O) until the volume is 1L, and the configuration is complete. Application of MOPSO: 1. Stable substrate solutions can be prepared under low-temperature biochemical working conditions of biological buffers, which are suitable for use in low-temperature working experiments; 1. Used as a buffer system for measuring the byproducts of biological treatment of marine crude oil; 2. To fix cells from urine sources, MOPSO ethanol buffer systems are usually prepared using MOPSO; 3. Can be used as one of the buffer components for carbon yeast extract culture medium and copper related analysis; 4. In the new liquid phase separation technology of capillary electrophoresis, MOPSO can be used as a carrier electrolyte; 5. For the preparation of glutathione synthase crystal, it acts as a buffer; 6. Some physicochemical research methods such as fluorescence spectroscopy, spectrophotometry, and isothermal titration calorimetry can be used in which MOPSO buffer can be used; 6. The interaction between the peptide skeleton of MOPSO and BSA can prevent the thermal denaturation of bovine serum albumin; 7. As one of the buffer components for copper correlation analysis. Hubei Xindesheng Material Technology Co., Ltd. is a source manufacturer of MOPSO buffering agents. It has 17 years of experience in research and development, production, and use, and has rich product knowledge. Currently, it produces and sells more than ten types of biological buffering agents, with a complete range of products. If you have purchasing needs, you can go to the official website of Desheng to inquire about the details or directly call to order. We look forward to your visit!

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