The enzymatic detection of homocysteine (Hcy) is a convenient and stable liquid dual reagent, which is very suitable for use with two reagent system analyzers. This testing method is fast, accurate, low-cost, and can effectively replace bulky and expensive immunochemical methods. This Hcy detection method can provide specific and convenient packaging for different instruments, thereby eliminating the inconvenience caused by the back and forth transfer of reagents when operating on different fully automated biochemical analyzers. This Hcy detection method has good correlation with high-performance liquid chromatography and immunochemical methods, is accurate and reliable, and the linear range can reach 3-50 μ Mol/L, coefficient of variation (CV) for differences within and between batches<7.5%. This Hcy detection method is significantly immune to interference from lipid and hemolytic substances, eliminating the "carry" interference of iron or lipase reagents in other methodologies. More importantly, this detection method is not interfered by endogenous cystathionides, making it more reliable for detecting Hcy in patients with kidney disease. Measurement principle: When measuring homocysteine content, oxidized Hcy is first reduced to free Hcy, and then reacts with the co substrate S-adenosylmethionine (SAM) under the action of Hcy methyltransferase (HMT) to generate methionine (Met) and S-adenosylhomocysteine (SAH). SAH was subsequently detected under the coupling action of SAH hydrolase (SAHH), adenosine deaminase (ADA), and glutamate dehydrogenase. SAH is hydrolyzed by SAHH to adenosine (Ado) and Hcy, forming an Hcy cycle that enters the initial, HMT catalyzed Hcy and co substrate SAM demethylation reaction. The formed Ado is immediately deammoniated to form inosine and ammonia. Finally, glutamate dehydrogenase (GLDH) catalyzes ammonia and α- The reaction between ketoglutaric acid and NADH to form NAD+.