Biological buffer MOPSO is an organic acid buffer with the molecular formula C7H15NO4S, a molecular weight of 223.26, and a pH range of 7.2-7.6. It can be used to buffer biochemical enzyme reactions and protein purification in biochemical experiments. This article will introduce the advantages of MOPSO and its application in biochemical experiments. Advantages of OPSO Compared with the commonly used buffering agents HEPES and Tris HCl in living organisms, MOPSO has the following advantages: 1. The buffering capacity of MOPSO is more stable, so it is less prone to pH fluctuations during the reaction process; 2. MOPSO has low ionic strength and high hydrophobicity, which will not affect the functions of many enzymes and proteins; 3. MOPSO has good protective effects on many important biological molecules, such as DNA, RNA, and enzymes; 4. MOPSO will not interact with some commonly used gel blockers, dyes and metal ions. Application of MOPSO in Biochemical Experiments 1. Buffer solution for enzyme reaction MOPSO can be used as an ideal buffer for various biochemical enzyme reactions, such as protein kinase, nucleotide phosphatase, esterase, isomerase, etc. In addition, MOPSO can also be used to catalyze the acetylation transfer reaction of deacetylated CoA. 2. Protein purification MOPSO can be used to purify proteins, which can effectively protect the structure and function of proteins and reduce the risks caused by changes in acidity and alkalinity. 3. RNA electrophoresis buffer MOPSO can serve as an RNA electrophoresis buffer. Because MOPSO can inhibit the electrophoretic migration of a small amount of DNA, it has little impact on RNA electrophoresis and can maintain RNA integrity, thus better separating RNA.