Blood coagulants are commonly used reagents in the laboratory to promote blood coagulation in vitro vacuum tubes. However, if the coagulant is not thoroughly mixed before use, it may have a negative impact on the experimental results, leading to misjudgment by medical staff, and also detrimental to the prevention and treatment of patients' diseases. matters needing attention 1. Before using a vacuum test tube for machine testing, it is necessary to ensure that the blood sample is thoroughly mixed with the reagent. If half of the sample solidifies and half does not, it may cause blockage of the analytical instrument and damage to blood cell components. 2. When using blood coagulants, operators should strictly adhere to operating procedures, including gently flipping the test tube to avoid violent shaking or reversing the reagent to prevent component damage. 3. Strictly follow the instructions: When using blood coagulants, it is important to strictly follow the instructions. Pay attention to storage conditions, usage methods, and expiration dates to ensure the quality and performance of the reagents. 4. Quality control and validation: During the experimental process, quality control and validation steps should be carried out to ensure that the effectiveness of blood coagulants meets expectations, and quality control samples or reference values can be used for validation.