Acridine ester NSP-DMAE-NHS, CAS 194357-64-7, is a luminescent reagent composed of acridine ester and a derivative of dimethylaminophenol (DMAE). DMAE can react with catalase to generate strong fluorescence signals. Due to the emitted fluorescence signals, many people currently use them as fluorescent probes to facilitate the detection of catalase activity in biological molecules. Due to the luminescent advantage of acridine ester, its application fields and effects are also very obvious. According to existing experimental research, it can be applied in chemiluminescence and immunoassay, as well as in nucleic acid and peptide detection, such as detecting catalase activity in tumor cells through a reagent kit and evaluating the functional status of immune cells. The accuracy and sensitivity of acridine ester NSP-DMAE-NHS have been fully demonstrated in these aspects.